Assessment of Green Manure Crops and their Impacts on Mineralizable Nitrogen and Changes of Nutrient Contents in the Soil

Thidar Hlaing *

Department of Agronomy, Yezin Agricultural University (YAU), Myanmar.

Kyi Moe

Department of Agronomy, YAU, Myanmar.

Ei Han Kyaw

Department of Agronomy, YAU, Myanmar.

Kyaw Ngwe

Department of Soil and Water Science, YAU, Myanmar.

Myat Moe Hlaing

Department of Plant Breeding, Physiology and Ecology, YAU, Myanmar.

Htay Htay Oo

Department of Agronomy, YAU, Myanmar.

*Author to whom correspondence should be addressed.


To evaluate the green manure crops those can promote soil properties and the ability of nitrogen (N) mineralization in paddy soil, a pot experiment was conducted at the Department of Agronomy, Yezin Agricultural University, Naypyidaw, Myanmar from April to August 2022. The experimental design was a randomized complete block design with three replications. Ten green manure crops were sown as treatments: soybean (Glycine max (L.) Merr), green gram (Vigna radiata L. Wildzek.), black gram (Vigna mungo L. Hepper.), cowpea (Vigna catjang), cowpea white (Vigna unguiculata (L.) Walp.), lab lab bean (Lablab purpureus (L.) Sweet.), mayflower bean (Phaseolus vulgaris L.), rice bean (Vigna umbellata), sunn hemp (Crotalaria juncea L.), and dhaincha (Sesbania bispinosa (Jacq.)). At the flowering stage, various green manures were incorporated into the soil, and their growth characters were measured along with the mineralizable N, total N, phosphorus (P2O5), and potassium (K2O) contents of the soil. The results indicated that rice bean, sunn hemp, and dhaincha had higher shoot and root length, shoot and root fresh weight, and dry weight compared to other varieties. In addition, they contain a higher percentage of total N content and total P2O5, and consequently, its C:N ratio was lower. After incorporating green manures into the soil, the mineralizable N increased gradually from 0 weeks to 10 weeks and then, the nutrient values decreased. Among green manures, sunn hemp, dhaincha, and rice bean were found to have higher mineralizable N (%) and applicable mineralized N than others. In conclusion, the incorporation of green manures into the soil improves the N, P2O5, and K2O contents of the soil, along with the mineralizable N content. Among green manure crops, dhaincha, rice bean, and sunn hemp were superior to other varieties due to higher biomass yield, mineralizable N (%), and chemical compositions, especially total N content.

Keywords: Green manures, soil nutrient contents, mineralizable nitrogen, biomass

How to Cite

Hlaing, T., Moe, K., Kyaw , E. H., Ngwe, K., Hlaing, M. M., & Oo , H. H. (2024). Assessment of Green Manure Crops and their Impacts on Mineralizable Nitrogen and Changes of Nutrient Contents in the Soil. Asian Soil Research Journal, 8(2), 29–38.


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Fu J., Wang ZQ, Yu LM, Wang XM, Yang JC. Effect of nitrogen rates on grain yield and some physiological traits of super rice. Chin J Rice Sci. (In Chinese with English abstract). 2014;28(4):391–400.

Pei PG, Zhang JH, Zhu LF, Hu ZH, Jin QY. Effects of straw returning coupled with N application on rice photosynthetic characteristics, nitrogen uptake, and grain yield formation. Chin J Rice Sci. (in Chinese with English abstract). 2015; 29(3):282–290.

Dal Ferro N, Charrier P, Marari F. Dual-scale micro-CT assessment of soil structure in a long-term fertilization experiment. Geoderma. 2013;204–205:8 4-93.

Pagliai M, Vignozzi N, Pellegrini S. Soil structure and the effect of management practices. Soil and Tillage Research. 2004; 79(2):131-143.

Korwar GR, Pratibha GH, Ravi V, Palanikumar D. Influence of organics and inorganics on growth, yield of aonla (Emblica officinalis) and soil quality in semi-arid tropics. Indian J Agric Sci. 2006;76(8):457–461.

Mohapatra A, Dash DK, Tripathy P. Comparative analysis of various organic amendments on tree growth and nut yield of cashew (Anacardium occidentale L.). Int J Agric Environ Biotechnol. 2016;9(2):225–230.

Bar AR, Baggie I, Sanginga N. The use of sesbania (Sesbania rostrata) and urea in lowland rice production in Sierra Leone. Agroforestry System. 2000;48(2):111-118.

Panneerselvam P, Manuel RI. Sustaining agriculture through green manuring. India’s National Newspaper; 2004.

Ohyama T, Ito M, Kobayashi K, Araki S, Yasuyoshi S, Sasaki O, Yamazaki T, Soyama K, Tanemura R, Tanemura Y, Ikarashi T. Analytical procedures of N, P, K contents and manure materials using H2SO4-H2O2 Kjeldahl digestion method. Bulletin of the Faculty of Agriculture, Niigata University. (in Japanese with English summary). 1991;43:111-120.

Cataldo DA, Schrader LE, Youngs VL. Analysis by digestion and colorimetric assay of total nitrogen in plant tissues high in nitrate. Crop Science. 1974;14:854–56.

Cataldo DA, Haroon M, Schrader LE., Youngs VL. Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Commun. Soil ScL Plant Anal. 1975;6:71-86.

Blake GR, Hartge KH. Bulk density. In: Klute A, Ed., Methods of soil analysis, part 1-Physical and mineralogical methods, 2nd Edition, agronomy monograph 9, American Society of Agronomy—Soil Science Society of America, Madison. 1986;363-38 2.

Smith JL, Doran JW. Measurement and use of pH and electrical conductivity for soil quality analysis. In J.W. Doran and A. J. Jones (ed.) Methods for assessing soil quality. SSSA Spec. Publ. 49. SSSA, Madison, WI. 1996;169–185.

Piper CS. Soil and plant analysis. Hans publisher, Botany; 1966.

Jason ML. Soil chemical analysis. Prentic-Hall, Inc., Englewood Cliffs, N.J; 1958.

Walkley A, Black I. A. An examination of the degtjareff method for determining soil organic matter and a proposed modification of the chromic Acid titration method. Soil Science.1934;37:29-38. 01000-00003

Bremner JM. Total nitrogen. In: Black CA et al., (ed.). Methods of soil analysis, Part 2. Agronomy 9 Am. Soc of Agron., Inc., Madison, Wis. 1965;1149-1178.

Olsen SR, Sommers LE. Phosphorus. In: Page AL et al. (ed.). Methods of soil analysis, Part 2. Chemical and microbial properties. 2nd ed. Agro. Monogr, 9. ASA and SSSA, Madison, WI. 1982;403-430.

Kudsen D, Peterson GA, Pratt PF, Lithium sodium and potassium. In: Page, A. L(Ed.), Method of soil analysis, Part 2. Chemical and microbiological properties, 2nd ed. Agronomy No. 9. ASA, SSAA, Madison, WI. 1982;225-246.

Lu HJ, Ye, ZQ, Zhang XL, Lin, XY, Ni WZ., Growth and yield responses of crops and macronutrient balance influenced by commercial organic manure used as a partial substitute for chemical fertilizers in an intensive vegetable cropping system. Phys Chem Earth Parts A/b/c. 2011;;36(9–11):387–394.

Becker M, Ladha JK, Ali M. Green manure technology: Potential, usage, and limitations. A case study for lowland rice.1 West Africa Rice. Development Association (WARDA), BP 2551, Bouakg, C6te d'lvoire, 2International Rice Research Institute (IRRI), PO Box 933, Manila, Philippines and 3Asian Vegetable Research and Development Center (AVRDC), PO Box 42, Shanhua, Tainan, Taiwan 74199, Plant and Soil. 1995;174: 181-194.

Cherr CM, Scholberg JMS, McSorley R. Green manure approaches to crop production. A synthesis. Agron. J. 2006;9 8(2):302–319.

Zubair M, Anwar F, Ashraf M, Ashraf A, Chatha SAS. Effect of green and farmyard manure on carbohydrates dynamics of salt-affected soil, J. Soil Sci. Plant Nutr. 2012;12(123):497–510.

Desaeger, J., Rao, M. R. The potential of mixed covers of Sesbania, Tephrosia, and Crotalaria to minimize nematode problems on subsequent crops. Field Crop. Res. 2001;70(2):111–125.

Maobe SN, Mburu LSMMWK, Ndufa JK, Mureithi JGCK, Gachene K, Okello JJ, Makini FW. Effect of mucuna green manure application rate on decomposition and soil available nitrogen under field conditions: I. During the season of incorporation. World J. Agric. Sci. 2011; 7:(4):430–438.

Parton W, Whendee LS, Ingrid CB, Leo G, Mark EH, William SC, Jennifer YK, Adair EC, Leslie AB, Stephen CH, Becky F. Global-Scale Similarities in Nitrogen Release Patterns During Long-Term Decomposition, Science (80). 2007;315 (5810):361–364.

Ha KV, Marschner P, Bünemann EK. Dynamics of C, N, P and microbial community composition in particulate soil organic matter during residue decomposition. Plant and Soil. 2008;303 :253–264.

Havstad LT, Aamlid TS, Henriksen TM. Decomposition of straw from herbage seed production: Effects of species, nutrient amendment and straw placement on C and N net mineralization. Acta Agriculturae Scandinavica Section B – Soil and Plant Science. 2010;60:57–68.

Buresh RJ, De Datta SK. Nitrogen dynamics and management of rice-legume cropping systems. Adv. Agron. 1991;45: 1-59.

Kumar S, Meena RS, Singh RK, Munir TM, Datta R, Danish S, Yadav GS, Kumar S. Soil microbial and nutrient dynam-ics under different sowings environment of Indian mustard (Brassica juncea L.) in rice based cropping system. Sci Rep. 2021a;11:5289. Available:

Singh I, Sheoran S, Kumar B, Kumar K, Rakshit S. Speed breeding in maize (Zea mays) vis-à-vis in other crops: status and pros-pects. Indian J. Agric. Sci. 2021;91 (9):1267–1273.

Jangir CK, Thakur A, Bijani H, Thakur P, Kumarc S, Meena RS, Bedwal S, Ranif K, Shuk-lag UN, Meenah AK, Purushottam D. Residual nitrogen for succeeding crops in legume-based cropping system; 2022. DOI: 10.1016/B978-0-323-85797-0.00024-0.